Heterozygote x Wild-type [C57BL/6NCrSlc] Heterozygote x Wild-type [C57BL/6NCrSlc] Lats2遺伝子のノックアウトマウス。エクソン1がPGK-neoカセットで置換されている。ホモマウスは胎生期致死。, true Hiroshi NOJIMA <a href='https://brc.riken.jp/mus/pcr01903'>Genotyping protocol -PCR-</a> 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J Biol Chem., 282, 19259-19271 (2007).<br>共同研究。 Developed by Norikazu Yabuta and Hiroshi Nojima, Research Institute for Microbial Diseases, Osaka University in 2002. D3 ES cells were used. The mutant mice were crossed to C57BL/6. 野島 博 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Biol Chem., 282, 19259-19271 (2007).The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Osaka Univ. Lats2遺伝子のノックアウトマウス。エクソン1がPGK-neoカセットで置換されている。ホモマウスは胎生期致死。 国立大学法人大阪大学 Cell Biology Research Tats2 heterozygous (He) mouse Tats2 heterozygous (He) mouse D3 [129S2/SvPas] B6;129S2-Lats2<tm1Noj> B6;129S2-Lats2<tm1Noj> Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Lats2 gene knockout mice. The first coding exon was replaced with a PGK-neo cassette. Homozygous mutant mice are embryonic lethal. mouse Lats2 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter) 大阪大学微生物病研究所・薮田紀一先生、野島 博先生(2002)。D3 ES細胞を用いて作出。C57BL/6と129の混合背景(B6セミコンジェニック)。 C (3-6 months) RBRC01903 C(3〜6か月)